Development of transduction layers for construction of biosensors used in food quality control, medical diagnostics and natural environmental monitoring


General Information:
International Project between Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn and Department of Chemistry University of Leuven

Proposal title:
Development of transduction layers for construction of biosensors used in food quality control, medical diagnostics and natural environmental monitoring

Number of Project:
679/N-BELGIA/2010/0

Start Date:
March 19th, 2010

Duration:
36 months

Short description of the proposal:

    We aim to study of novel electro-mediator layers based on homo and hetero derivatives of metal oligomers and electrochemical characteristic of it. The best one will be used as molecular nano-unit to perform the construction of biosensor for monitoring of food and medical diagnosis. Such nano-units are especially interesting and could be used for construction of enzymatic biosensors and sensors with redox-active proteins.

    Also we would like to analyze changes of transductive properties of proposed layers depending on length of oligopolymers investigated metallocomplexes, its structure, number of metal in oligopolymer and influence of many metals in oligopolymer. Fundamental unit for construction of nano-units will be olipolymers of metallocomplex (Cu, Co, Ni, Fe) of dipyromethene, porphyrin and corrole.

    As a part of this project we would like to study the methods of immobilization of bioreceptors on the surface of electrodes using nano-units base on metaloorganic complexes oligomers.

Specific tasks include:

  • Synthesis of dipyromethene, porphyrin and corrole derivatives
  • Synthesis of dipyromethene, porphyrin and corrole derivatives with Cu(II), Co(II) and Ni(III).
  • Methods of immobilization of dipyromethene, porphyrin and corrole derivatives on the surface of electrodes.
  • Methods of immobilization of complexes of dipyromethene, porphyrin and corrole derivatives with Cu(II), Co(II) and Ni(III) on the surface of electrodes – electrochemical characterization of nano-joints.
  • Methods of formation of complexes of dipyromethene, porphyrin, corrole derivatives with Cu(II), Co(II) and Ni(III) on the surface of electrodes – electrochemical characterization of nano-joints.
  • Methods of immobilization of selected antibodies, enzymes, ssDNA and redoxactive proteins on the surfaces of selected nano-joints.
  • Application of selected nano-joints for the development of enzyme biosensors for control of food products and medical samples.
  • Application of selected nano-joints for the development of genosensorsfor control of  food products and medical samples.
  • Application of selected nano-joints for the development of biosensors based on redox active proteins for control of food products and medical samples.
  • Application of selected nano-joints for the development of immunosensors for control of  food products and medical samples.

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