Study of the interactions between V domain of Receptor for Advanced Glycation End Products (RAGE) and Aβ oligomers in the presence of selected alkaloids
The receptor for advanced glycation endproducts (RAGE) has been shown to interact with A-beta and to modulate A-beta transport across the blood-brain barrier. Some evidences, recent published, suggest that RAGE is a key player in the signaling pathways triggered by Abeta and S100 proteins in AD. The objective of our research is establishing the relationship between the structure of selected alkaloids and their influence on the kinetics of interactions between RAGE and A-beta peptide.
This research will be performed with electrochemical techniques, surface plasmon resonance, and atomic force microscopy.
Study of the interactions between Janus kinase (JAKs) and potential inhibitors
The kinases play the critical roles in fundamental cellular processes, such as: cell cycle, cell division, differentiation and apoptosis. The searching of their efficient inhibitors- potential anticancer drugs- is very intensively developing research field. We are working on the fabrication of analytical tools suitable for screening of the interactions between his-tagged kinases and their potential inhibitors. The redox active monolayer was deposited onto gold electrode by progressive modification of thiol derivative of compounds chelating transition metal cations are applied for oriented immobilization of his-tagged kinase protein. The biosensors obtained are used for study of interactions between his-tagged kinase protein immobilized on the redox active surface and its inhibitors present in the aqueous solution. Such system mimics well physiological conditions.