The development of electrochemical immunosensors and genosensors for the detection of Plum Pox Virus (PPV) and Prunus Necrotic Ringspot Virus (PNRSV) in plant material


Duration: September 1, 2009 – July 31, 2012

Number of the project: N R12 0023 06
The project was supported by Polish Ministry of Science and Higher Education

Contractors:

  1. Department of Biosensors of Polish Academy of Sciences in Olsztyn
    • Prof. Jerzy Radecki
    • Prof. Hanna Radecka
    • M. Sc. Urszula Jarocka
    • M. Sc. Kamila Malecka
  2. Department of Physiology and Biochemistry, Research Institute of Horticulture in Skierniewice
    • Prof. Lech Michalczuk
    • Ph. D. Tadeusz Malinowski

The aim of the project:

The aim of this project is to design and obtain electrochemical immuno- and genosensors allowing the detection of Plum Pox Virus (PPV) and Prunus Necrotic Ringspot Virus (PNRSV) in the plant material (leaf extract).
After appropriate modifications, these sensors can be used for the detection and identification of other plant viruses with similar construction.

The Plum Pox Virus is most dangerous viral disease of plants of the genus Prunus (plums, peaches and apricots). PPV is spread by vegetative propagation and  transmitted by aphids. Symptoms are visible only after 3 years of infection. Harmfulness of the disease is associated with the weakening of the growth of infected trees, reduction in yield and deterioration of its quality, premature fruit drooping and increased sensitivity to stress factors. Trees susceptible varieties may die.

Prunus Necrotic Ringspot of Stone Fruit Trees is one of the most dangerous viral diseases of cherries. The virus is transmitted from pollen and seeds. Infected trees grow very weakly, and the yield is often lower by 80%.

On 26 May and 27 October 2011, working meeting related to the implementation of the grant took place at the Institute of Animal Reproduction and Food Research. The purpose of the meeting was to discuss current experiments and to plan further ones.

Participants of the meeting:

  1. Department of Biosensors of Polish Academy of Sciences in Olsztyn
    • Prof. Jerzy Radecki
    • Prof. Hanna Radecka
    • M. Sc. Urszula Jarocka
    • M. Sc. Kamila Malecka
  2. Department of Physiology and Biochemistry, Research Institute of Horticulture in Skierniewice
    • Prof. Lech Michalczuk
    • Ph. D. Tadeusz Malinowski

 

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